Part:BBa_K091152:Design

pLas' Testing Construct D

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 1190
  Illegal AgeI site found at 1387
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 2591
  

Design Notes

pLas' Testing Construct D has the coding region for LuxR preceding the region for LasR, but the order of these regions is reversed in pLas' Testing Construct C

Source

The sources of the quorum sensing components are the modified lasI regulatory region in P. aeruginosa and the lux components from V. fisheri.